Laboratory Test Detail

Last Updated: September 21, 2019 @ 9:13 am

91700

Leukemia/Lymphoma Phenotyping [Flow Cytometry]

Specimen:Collect:
  • Bone Marrow in Lavender (EDTA)

Also Acceptable:
  • Whole blood in Green Top (Na Heparin)
  • Whole blood in Lavender (EDTA)
  • Whole blood in Pink Top (EDTA)
  • Body Fluid in Sterile Specimen Container
  • Tissue in Body Fluid in Sterile Specimen Container
Submit:
  • 1 mL (Min:0.5 mL) Bone Marrow in 4 mL Lavender (EDTA). Submit Ambient.

Also Acceptable:
  • 5 mL (Min:1 mL) Whole blood in 4 mL Green Top (Na Heparin). Submit Ambient.
  • 5 mL (Min:1 mL) Whole blood in 4 mL Lavender (EDTA). Submit Ambient.
  • 10 mL (Min:3 mL) Body Fluid in Sterile Specimen Container. Submit Refrigerated.
  • One Tissue in Sterile Specimen Container. Submit Refrigerated.
  • 5 mL (Min:1 mL) Whole blood in Pink Top (EDTA). Submit Refrigerated.
Special Handling:
  • Specimen Preparation: Transport 1 mL heparinized bone marrow (Min: 0.5 mL*) OR 5 mL whole blood (Min: 1mL*) OR 100 mg fresh tissue suspended in tissue culture media (e.g., RPMI 1640) (Min: 100 mg*) OR 10-100 mL fresh fluid (Min: 3 mL*).
    *Minimum volume is dependent on cellularity.
    Storage/Transport Temperature: Specimen should be received within 24 hours of collection for optimal cell viability. Bone marrow or whole blood: Room temperature. Also acceptable: Refrigerated.
    Tissue or fluid: Refrigerated.
    Remarks: A minimum of 10,000 viable cells is required for flow cytometry phenotyping of samples containing a very limited number of markers (may also be called antibodies or antigens). For low-count specimens, supplying clinical and diagnostic information is especially important to help ensure that the most appropriate marker combinations are evaluated before the specimen is depleted of cells.
    Bone marrow or whole blood: Provide specimen source, CBC, Wright stained smear, clinical history, differential diagnosis, and any relevant pathology reports.
    Tissue or fluid: Provide specimen source, clinical history, differential diagnosis, and any relevant pathology reports.
    Follow up: If previous leukemia/lymphoma phenotyping was performed at another lab, the outside flow cytometry report and histograms (if possible) should accompany the specimen.

    Charges apply per marker.
Rejection Criteria:
  • Clotted Specimen
  • Hemolyzed specimens
  • Specimen submitted frozen
Stability:Ambient: 2 Day(s); Refrigerated: 2 Day(s); Frozen: Unacceptable; Incubated: Unacceptable
Methodology:
  • Flow Cytometry
Performed:Sun-Sat
Reported:2-3 Day(s)
CPT Codes:
  • 88184
Interpretive Data:Please see report for interpretive data.
Components:
  • 91701 - Number of Markers
  • 91727 - SOURCE
  • 91729 - IMPRESSION
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