Methotrexate, Serum or Plasma

Specimen:

Collect

  • One Red Top

Also Acceptable:
  • One Green Top (Na Heparin)
  • One Lavender (EDTA)
  • One Pink Top (EDTA)

Submit

  • 1 mL (Min:0.3 mL) Serum in Standard Transport Tube. Submit Ambient. Submit in a Standard Transport Tube.

Also Acceptable:
  • 1 mL (Min:0.3 mL) Plasma in Standard Transport Tube. Submit Ambient. Submit in a Standard Transport Tube.
  • 1 mL (Min:0.3 mL) Plasma in Standard Transport Tube. Submit Ambient. Submit in a Standard Transport Tube.
  • 1 mL (Min:0.3 mL) Plasma in Standard Transport Tube. Submit Ambient. Submit in a Standard Transport Tube.

Special Handling

  • Separate aliquot required for each frozen test ordered

Rejection Criteria

  • Use of separator tubes

Stability

Ambient: 2 Week(s); Refrigerated: 2 Week(s); Frozen: 2 Week(s); Incubated: Unacceptable

Methodology

  • Liquid Chromatography Tandem Mass Spectrometry

Performed

Varies

Reported

7-9 Day(s)

CPT Codes

  • 80204

Components

  • 91164 - METHOTREXATE, SERUM OR PLASMA
Last Updated: October 8, 2025 @ 9:13 am

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Common Lab Definitions

  • Serum 
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  • STT