Laboratory Test Detail

Last Updated: November 21, 2019 @ 10:13 am

2776

Anti-Neutrophil Cytoplasmic Antibodies (ANCA) IFA and MPO/PR3 Antibodies

Specimen:Collect:
  • One SST

Also Acceptable:
  • One Red Top
Submit:
  • 2 mL (Min:1 mL) Serum . Submit Frozen. Submit in a Standard Transport Tube.
Rejection Criteria:
  • Grossly Hemolyzed Samples
  • Grossly Lipemic Samples
  • Heat inactivated
  • Microbially Contaminated
  • Particulate matter
Stability:Ambient: 8 Hour(s); Refrigerated: 2 Day(s); Frozen: 1 Month(s); Incubated: Unacceptable
Methodology:
  • Enzyme-Linked ImmunoSorbent Assay (ELISA)
  • Indirect Immunofluorescent Assay
Performed:Tuesday, Thursday
Reported:3-4 Day(s)
Positive results will be titered and reported next day.
CPT Codes:
  • 83520x2
  • 86255
Interpretive Data:Please see report for interpretive data.

Anti-Neutrophil Cytoplasmic Antibodies (ANCA) are generally found in the serum of patients with ANCA-associated vasculitis or other autoimmune disease.

Myeloperoxidase and Proteinase 3 Reference Ranges:
<21 Units Negative
21-30 Units Weak Positive
>30 Units Moderate to Strong Positive

ELISA method for ANCA correlates with Indirect Immunofluoresence as follows-
Myeloperoxidase = P-ANCA (Perinuclear Staining)
Proteinase 3 = C-ANCA (Cytoplasmic Staining)
Components:
  • 2781 - ANCA IFA TITER
  • 2783 - ANCA IFA PATTERN
  • 2277 - MYELOPEROXIDASE
  • 2279 - PROTEINASE 3
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