Specimen:
Collect
- One SST
Also Acceptable:
- One Red Top
Submit
- 2 mL (Min:1 mL) Serum . Submit Frozen. Submit in a Standard Transport Tube.
Rejection Criteria
- Grossly Hemolyzed Samples
- Grossly Lipemic Samples
- Heat inactivated
- Microbially Contaminated
- Particulate matter
Stability
Ambient: 8 Hour(s); Refrigerated: 2 Day(s); Frozen: 1 Month(s); Incubated: Unacceptable
Methodology
- Enzyme-Linked ImmunoSorbent Assay (ELISA)
- Indirect Immunofluorescent Assay
Performed
Tuesday, Thursday
Reported
3-4 Day(s)
Positive results will be titered and reported next day.
Positive results will be titered and reported next day.
CPT Codes
- 83520x2
- 86255
Interpretive Data
Please see report for interpretive data.
Anti-Neutrophil Cytoplasmic Antibodies (ANCA) are generally found in the serum of patients with ANCA-associated vasculitis or other autoimmune disease.
Myeloperoxidase and Proteinase 3 Reference Ranges:
<21 Units Negative
21-30 Units Weak Positive
>30 Units Moderate to Strong Positive
ELISA method for ANCA correlates with Indirect Immunofluoresence as follows-
Myeloperoxidase = P-ANCA (Perinuclear Staining)
Proteinase 3 = C-ANCA (Cytoplasmic Staining)
ANCA performed using Indirect Immunofluorescent Assay (IFA).
Anti-Neutrophil Cytoplasmic Antibodies (ANCA) are generally found in the serum of patients with ANCA-associated vasculitis or other autoimmune disease.
Myeloperoxidase and Proteinase 3 Reference Ranges:
<21 Units Negative
21-30 Units Weak Positive
>30 Units Moderate to Strong Positive
ELISA method for ANCA correlates with Indirect Immunofluoresence as follows-
Myeloperoxidase = P-ANCA (Perinuclear Staining)
Proteinase 3 = C-ANCA (Cytoplasmic Staining)
ANCA performed using Indirect Immunofluorescent Assay (IFA).
Components
- 2781 - ANCA IFA TITER
- 2783 - ANCA IFA PATTERN
- 2277 - MYELOPEROXIDASE
- 2279 - PROTEINASE 3
Last Updated: May 8, 2025 @ 9:13 am
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