Vitamin B12

Specimen:

Collect

  • One SST

Also Acceptable:
  • One Green Top (Na Heparin)
  • One Lavender (EDTA)
  • One Pink Top (EDTA)
  • One Red Top

Submit

  • 1 mL (Min:0.5 mL) Serum . Submit Refrigerated.

Also Acceptable:
  • 1 mL (Min:0.5 mL) Plasma . Submit Refrigerated.

Special Handling

  • Fasting Specimen is Preferred
  • For patients receiving therapy with high biotin doses (>5 mg/day), no laboratory test specimen should be collected until at least 8 hours after the last biotin administration.
  • Minimize air exposure
  • Protect from Light
  • Separate from cells within 2 hours of collection
  • SST collection stable for 24 hours.

Rejection Criteria

  • Grossly Hemolyzed Samples
  • Heat inactivated

Stability

Ambient: 2 Hour(s); Refrigerated: 2 Day(s); Frozen: 2 Month(s); Incubated: Unacceptable

Methodology

  • Electrochemiluminescence Immunoassay (ECLIA)

Performed

Mon-Fri

Reported

1-3 Day(s)

CPT Codes

  • 82607

Interpretive Data

General Reference Range : 232-1245 pg/ml

Biotin in specimens taken from patients on high-dose biotin therapy or supplements may intefere with this test and cause inaccurate test results. It is recommended that for patients receiving therapy with high biotin doses (> 5 mg/day), no laboratory test specimen should be collected until at least 8 hours after the last biotin administration.

Components

  • 2126 - VITAMIN B12
Last Updated: April 22, 2025 @ 9:13 am

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Common Lab Definitions

  • Serum 
  • Plasma 
  • STT