94038 Leukemia/Lymphoma Phenotype

Specimen:

Collect:

One 4 mL Lavender (EDTA)

Also Acceptable
One 4 mL Green Top (Na Heparin)
Bone Marrow
CSF
Pleural Fluid
Synovial Fluid
Vesical Fluid
Body Fluid

Submit:

4 mL (Min:3 mL) Blood in 4 mL Lavender (EDTA). Submit Ambient. Submit in a Standard Transport Tube.

Also Acceptable
4 mL (Min:1 mL) Bone Marrow. Submit Ambient. Submit in a Standard Transport Tube.
Body Fluid in Sterile Specimen Container. Submit Refrigerated. Submit in a Standard Transport Tube.

Special Handling:

PREPARE AND SHIP TO LAB IMMEDIATELY.
Instructions:
Peripheral blood: One green, or lavender-top (sodium heparin or EDTA) tube. A minimum of 3 mL is required. The tube must be kept at room temperature and shipped to the lab immediately.

Bone marrow: A minimum of 1 mL (with a maximum of 4 mL to prevent hemodilution of bone marrow) submitted in a green, or lavender-top (sodium heparin or EDTA) tube. The tube must be kept at room temperature and shipped immediately.

Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (Tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) . Ship tissue in sterile plastic container with RPMI 1640 enriched with FBS (10%FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.

Rejection Criteria:

Specimens submitted without adequate cells
Specimens submitted in fixative

Stability:

Ambient: 3 Day(s); Refrigerated: Unacceptable; Frozen: Unacceptable; Incubated: Unacceptable

Methodology:

Flow Cytometry

Performed:

Sun-Sat

Reported:

3-4 Day(s)
Sets up 7 days a week.

CPT Codes:

88184	88189
88185x21