94015 Hepatitis C Genotype

Specimen:

Collect:

One 4.5 mL PPT White Top (EDTA)

Also Acceptable
One 7.5 mL SST
One 4 mL Lavender (EDTA)

Submit:

2 mL (Min:0.6 mL) Plasma. Submit Frozen. Submit in a Standard Transport Tube.

Also Acceptable
2 mL (Min:0.6 mL) Serum. Submit Frozen. Submit in a Standard Transport Tube.

Special Handling:

Avoid Repeated Freeze/Thaw Cycles
Critical Frozen
Separate aliquot required for each frozen test ordered
Separate from cells ASAP
Transport Temperature: Frozen. Do Not Thaw.
This test may be unsuccessful if the HCV RNA viral load is less than 1,000 HCV RNA copies per mL of plasma.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant; either 0.15% solution v/v final EDTA K 3 (standard EDTA tube) or 9 mg spray-dried EDTA K 2 (Plasma Preparation Tube or PPT tube with plasma separator-gel, preferred). Blood collected in tubes containing ACD anticoagulant are acceptable but will yield results approximately 15% lower when compared to EDTA tubes due to the dilution effect of the 1.5 mL of anticoagulant used in the tube. Blood collected in tubes with heparin anticoagulant are unsuitable for this test. Store whole blood at room temperature and separate plasma from cells within 6 hours of collection. Transfer plasma to sterile, plastic, screw-capped aliquot tubes and store at -18°C or colder. Do not clarify plasma by filtration or further centrifugation. Avoid repeated freezing and thawing of specimen.
Note: If blood is collected in a PPT tube, centrifuge within 6 hours of collection as before, but it is not necessary to transfer the plasma to aliquot tubes. Following centrifugation, a gel barrier maintains separation of plasma from cellular components during specimen transport and storage, and unlike standard VACUTAINER Brand blood collection tubes, the PPT tube is plastic and hence the plasma can be shipped and stored frozen in the original tube.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum from cells within 6 hours of collection.

Rejection Criteria:

Thawed plasma
Unspun PPT tubes

Stability:

Ambient: 3 Hour(s); Refrigerated: 7 Day(s); Frozen: 3 Month(s); Incubated: Unacceptable

Methodology:

Multi-probe Reverse Hybridization

Performed:

Reported:

3-4 Day(s)
Sets up 5 days a week.

CPT Codes:

87902