91263 Platelet Antibody, Indirect

Specimen:

Collect:

One 10 mL Red Top

Also Acceptable
One 4 mL Lavender (EDTA)

Submit:

1 mL (Min:0.8 mL) Serum. Submit Frozen. Submit in a Standard Transport Tube.

Also Acceptable
1 mL (Min:0.8 mL) Plasma. Submit Frozen. Submit in a Standard Transport Tube.

Special Handling:

Separate from cells ASAP
For Newborns, less than 30 days old, samples should be collected from mother.

Rejection Criteria:

Grossly Lipemic Samples
Hemolyzed specimens
Use of separator tubes

Stability:

Ambient: Unacceptable; Refrigerated: 2 Day(s); Frozen: 1 Month(s); Incubated: Unacceptable

Methodology:

Enzyme-Linked ImmunoSorbent Assay (ELISA)

Performed:

Mon-Fri

Reported:

2-4 Day(s)

CPT Codes:

86022 - Antibody ID, Platelet

Interpretive Data:


This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of ITP. This test will detect both allo and autoantibodies, but will not distinguish between them. This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [P1a1 and P1A2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA CLass I antigens (HLA-A-B). Testing for neonatal alloimmune thrombocytopenia should be performed using maternal serum, since platelet antibody may not be detected in neonatal serum. False negative results are common in infant samples.