91086      Entamoeba histolytica, Antibody, IgG
Specimen:
  Collect: One 7.5 mL SST  
  Submit: 1 mL (Min:0.5 mL) Serum. Submit Refrigerated. Submit in a Standard Transport Tube.

Also Acceptable
1 mL (Min:0.5 mL) Plasma. Submit Refrigerated. Submit in a Standard Transport Tube. 
  Special Handling: Avoid Repeated Freeze/Thaw Cycles
Separate from cells ASAP
If acute and convalescent samples being submitted, label as such. Convalescent sample must be received in lab within 30 days.  
  Rejection Criteria: Grossly Lipemic Samples
Hemolyzed specimens 
  Stability: Ambient: 2 Day(s); Refrigerated: 2 Week(s); Frozen: 12 Month(s); Incubated: Unacceptable 
Methodology: Enzyme-Linked ImmunoSorbent Assay (ELISA) 
Performed: Tuesday, Friday 
Reported: 3-5 Day(s) 
CPT Codes:
86753 - Protozoa Antibody
Interpretive Data:
Reference Interval:
0.79 IV or less: Negative - No significant level of detectable E.histolytica IgG antibody
0.80-1.19 IV: Equivocal - Repeat testing in 10-14 days maybe helpful
1.20 IV or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection