| Specimen: |
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Collect: |
One 7.5 mL SST
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Submit: |
1 mL (Min:0.5 mL) Serum. Submit Refrigerated. Submit in a Standard Transport Tube.
|
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Special Handling: |
Allow specimen to clot completely at room temperature
Avoid Repeated Freeze/Thaw Cycles
Separate from cells ASAP |
| |
Rejection Criteria: |
Grossly Hemolyzed Samples
Grossly Lipemic Samples |
| |
Stability: |
Ambient: 8 Hour(s); Refrigerated: 2 Day(s); Frozen: 1 Month(s); Incubated: Unacceptable |
| Methodology: |
Enzyme-Linked ImmunoSorbent Assay (ELISA) |
| Performed: |
Tuesday, Thursday |
| Reported: |
2-5 Day(s) |
| CPT Codes: |
| 86789 - West Nile Ab, IgG |
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| Interpretive Data: |
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West Nile IgG Reference Range:
<1.30 NEGATIVE
1.30-1.50 EQUIVOCAL (Suggest repeat testing in 2 weeks)
>1.50 POSITIVE
This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgG and IgM in serum samples in which there is a clinical suspicion of West Nile virus infection. This test should not be used solely for quantitative purpose, nor should the results be used without correlation to clinical history or other data. Because other members of the Flaviviridae family, such as St. Louis encephalitis virus, show extensive cross-reactivity with West Nile virus, presumptive positive results should be confirmed using tests such as the plaque reduction neutralization test.
IgG assay cross-reactivity has been noted with some specimens containing antibody to CMV and bunyavirus, e.g., LaCrosse virus.
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