2226      Insulin Tolerance, 2 Hour
Specimen:
  Collect: Five 7.5 mL SST

Also Acceptable
Five 4 mL Green Top (Li Heparin) 
  Submit: Five 1 mL (Min:0.5 mL) Serum. Submit Refrigerated. Submit in a Standard Transport Tube.

Also Acceptable
Five 1 mL (Min:0.5 mL) Plasma. Submit Refrigerated. Submit in a Standard Transport Tube. 
  Special Handling: Allow specimen to clot completely at room temperature
Separate from cells ASAP
Draw fasting specimen, then give 75gm glucola. At 1/2 hour draw "1/2 HR" post sample. At 1 hour draw "1 HR" post sample. At 1 1/2 HR draw "1 1/2 HR" post sample. At 2 hour draw "2 HR" post sample.
Label tubes "Fasting", "1/2 HR", "1 HR", "1 1/2 HR", and "2 HR".
Freeze specimens if delayed more than 48 hours before testing. 
  Rejection Criteria: EDTA plasma
Hemolyzed specimens 
  Stability: Ambient: Unacceptable; Refrigerated: 2 Day(s); Frozen: 3 Month(s); Incubated: Unacceptable 
Methodology: Colorimetric; Kinetic 
Performed: Mon-Fri 
Reported: 1-3 Day(s) 
CPT Codes:
83525 X5 - Insulin, Total
Interpretive Data: Please see report for interpretive data.
Components:
2227 - INSULIN, FASTING2230 - INSULIN, 30 MIN
2256 - INSULIN, 60 MIN2257 - INSULIN, 90 MIN
2258 - INSULIN, 120 MIN